Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats
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Research Article
VOLUME: 5 ISSUE: 3
P: 99 - 108
December 2017

Investigation of Protective Effect of Hypericum Perforatum on Ethanol-Induced Acute Gastric Mucosal Injury in Rats

Namik Kemal Med J 2017;5(3):99-108
1. Namık Kemal Üniversitesi, Sağlık Yüksekokulu, Acil Yardım ve Afet Yönetimi Bölümü, Tekirdağ, Türkiye
2. Çanakkale Onsekiz Mart Üniversitesi, Tıp Fakültesi, Fizyoloji ABD, Çanakkale, Türkiye
3. Namık Kemal Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya ABD, Tekirdağ, Türkiye
No information available.
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Received Date: 12.09.2017
Accepted Date: 02.11.2017
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ABSTRACT

Conclusion:

It was concluded that H. perforatum and olive oil may represents a potential therapeutic option to reduce the risk of gastric mucosal ulceration.

Results:

Elevated ulcer index and increased IL-6, iNOS, TUNEL and MDA levels were detected in the ethanol-induced gastric injury model (grup II). Pre-treatment of H. perforatum and olive oil significantly attenuated the gastric lesions as compared to the group II.

Material and Methods:

Forty male Wistar albino rats were used in this study. Rats were divided four group, randomly. Group I (Control, n=10), Group II (Ethanol, n=10, 1 ml ethanol, orally, 90 min) Group III (H. perforatum + ethanol, 1 ml H. perforatum, 120 min; 1 ml ethanol, 90 min, orally), Group IV (Olive oil + ethanol, 1 ml olive oil, 120 min; 1 ml ethanol, 90 min, orally). Gastric ulcer score was determined in gastric mucosa by morphometrically. Histopatological staining, Immunohistochemical iNOS and PCNA staining, apoptotic TUNEL staining, gastric IL-10 and IL-6 expression (ELISA) and SOD, MDA, CAT levels were assessed in gastric tissue.

Aim:

This study was conducted to evaluate the antiapoptototic and antiinflammatory effect of Hypericum perforatum in the rats induced gastric ulcer by absolute ethanol.

Keywords:
Gastric ulcer, Hypericum perforatum, iNOS, TUNEL, Interleukin-6, Interleukin-10