ABSTRACT

Aim:

Coronary artery disease (CAD) is a pathological process characterized by atherosclerotic plaque accumulation in the epicardial arteries. Inflammation and high lipid levels play a role in pathological changes in atherosclerosis. Besides traditional risk factors, genetic factors such as single nucleotide polymorphism (SNPs) can be involved in disease process. In this study, we aimed to evaluate the effects of oxidized low density lipoprotein receptor 1 (OLR1) 3’UTR188C>T gene polymorphism, C-reactive protein (CRP), and lipid status in patients with coronary artery bypass grafting (CABG).

Materials and Methods:

The study population consisted of 109 CAD patients who had undergone CABG, and 127 healthy controls. The OLR1 3’UTR188C>T polymorphism was genotyped using PCR-RFLP technique. Serum CRP, high-density lipoprotein-cholesterol (HDL-C), and low-density lipoprotein-cholesterol (LDL-C) levels were measured with an automatic biochemistry analyzer.

Results:

The distribution of the OLR1 3’UTR188C>T genotypes and alleles did not differ significantly between CAD patients with CABG and controls. Serum CRP levels were increased in patients compared to the control group (p<0.001), but HDL-C, and LDL-C levels were not different between two groups. Traditional risk factor such as cigarette smoking, alcohol use, family history, diabetes mellitus and hypertension were increased in patients compared to the control group (p<0.001, for each). The CRP levels were higher in patients with the TT, CT, and CC genotypes than in controls with the same genotypes (p<0.001, p<0.01, and p<0.05, respectively).

Conclusion:

OLR1 3’UTR 188C>T polymorphism may not be involved in susceptibility to atherosclerosis. However, traditional risk factors in atherosclerosis such as smoking, alcohol consumption, family history, hypertension, diabetes mellitus, and circulating CRP levels were increased in our CABG population. The evaluation of OLR1 3’UTR188C>T and different OLR1 SNPs may be useful for their single and combined effects in atherosclerosis.

INTRODUCTION

Coronary artery bypass grafting (CABG) is a surgical procedure in which vessels are used as grafts to bypass coronary arteries that are partially or completely occluded by atherosclerotic plaque¹. Atherosclerosis is a disease characterized by the accumulation of lipids, fibrous elements, and calcification in the arteries. This process starts with the activation of the endothelium, and then a series of events that occur with the activation of the inflammatory response lead to vasoconstriction and atheroma plaque formation².

Many studies have tried to explain the biological and genetic basis of atherosclerosis^3-6. Although experimental studies are helping to unravel the pathophysiology of atherosclerosis, clinical gaps remain⁵. Various methods used to study the genetic factors involved in chronic diseases such as atherosclerosis have focused on the genetic basis in the development of the disease. However, in most chronic diseases, multiple genes acting under the influence of several environmental factors have been used to determine the development of diseases such as atherosclerosis. The identification of these genes essentially followed a candidate gene approach. Based on the understanding of the pathogenesis of atherosclerosis genes involved in lipid mobilization, for example, the association with inflammation and endothelial function, the presence of atheroma or cardiovascular events has been studied⁷. It has been reported that genes associated with the coronary artery disease (CAD) process can be divided into three categories: disease-causing genes, susceptibility genes, and disease-associated genes^8,9.

The human gene encoding the oxidized low density lipoprotein receptor 1 (OLR1) maps to chromosome 12p13.1-p12.3 and consists of 6 exons interrupted by 5 introns. The OLR1 gene is a good candidate gene for cardiovascular disease because of its location in a chromosomal region often associated with cardiovascular disorders and the biochemical role of its product in lipid metabolism pathways^10,11. Six non-coding single-nucleotide polymorphisms (SNPs) in the 3’-terminal portion of OLR1 have been reported to be involved in alternative splicing of exon 5 and expression of the LOXIN transcript variant lacking exon 5 of OLR1. These 6 noncoding polymorphisms, including in 3’UTR (188C>T), intron 5 (IVS5-27G>T and IVS5-70A>G) and intron 4 (IVS4-14A>G, IVS4+27G>C, IVS4-73C>T), are located in a haplotype block region and affect the risk of developing CAD by changing LOXIN expression^12,13. Studies have reported that OLR1 may play an important role in the pathophysiology of atherosclerosis and thrombosis^13,14. 188C>T, a polymorphism in the 3’-UTR of the OLR1 gene, has been found to be associated with CAD^13,15, but there are also studies reporting that this polymorphism is not altered in vascular diseases^16-18. It has also been reported that C-reactive protein (CRP), which has proinflammatory effects, may play a role in the pathogenesis of CAD by showing proatherogenic and prothrombotic effects on vascular cells¹⁹. Considering this information, we aimed to investigate the OLR1 188C>T polymorphism and serum CRP and lipid levels in patients who underwent CABG in the Turkish population.

GİRİŞ

Koroner arter bypass grefti (KABG), aterosklerotik plak tarafından kısmen veya tamamen tıkanmış olan koroner arterleri bypass etmek için damarların greft olarak kullanıldığı cerrahi bir prosedürdür¹. Ateroskleroz, arterlerde lipitlerin, fibröz elementlerin birikmesi ve kalsifikasyonla karakterize bir hastalıktır. Bu süreç endotelin aktivasyonu ile başlamakta ve ardından enflamatuvar yanıtın aktivasyonu ile ortaya çıkan bir dizi olay vazokonstriksiyona ve aterom plağı oluşumuna yol açmaktadır².

Birçok çalışma aterosklerozun biyolojik ve genetik temelini açıklamaya çalışmıştır^3-6. Deneysel çalışmalar aterosklerozun patofizyolojisinin aydınlatılmasına yardımcı olmasına rağmen klinik boşluklar devam etmektedir⁵. Ateroskleroz gibi kronik hastalıklarda rol oynayan genetik faktörleri incelemek için kullanılan çeşitli yöntemler, hastalığın gelişimindeki genetik temele odaklanmıştır. Ancak ateroskleroz gibi çoğu kronik hastalıkta hastalıkların gelişimini belirlemek için çeşitli çevresel faktörlerin etkisi altında birden fazla etki eden gen araştırılmıştır. Bu genlerin tanımlanması aday gen yaklaşımını takip etmiştir. Lipid mobilizasyonunda yer alan ateroskleroz genlerinin patogenezinin anlaşılmasına dayanarak, örneğin enflamasyon ve endotel fonksiyonu ile ilişki, aterom veya kardiyovasküler olayların varlığı incelenmiştir⁷. Koroner arter hastalığı (KAH) süreciyle ilişkili genlerin üç kategoriye ayrılabileceği rapor edilmiştir: hastalığa neden olan genler, duyarlılık genleri ve hastalıkla ilişkili genler^8,9.

Oksitlenmiş düşük yoğunluklu lipoprotein reseptörü 1’i (OLR1) kodlayan insan geni, 12p13.1-p12.3 kromozomuyla eşleşmekte ve 5 intron tarafından kesintiye uğrayan 6 eksondan oluşmaktadır. OLR1 geni, sıklıkla kardiyovasküler bozukluklarla ilişkili kromozomal bölgedeki konumu ve ürününün lipid metabolizma yollarındaki biyokimyasal rolü nedeniyle kardiyovasküler hastalık için iyi bir aday gendir^10,11. Altı kodlamayan tek nükleotid polimorfizmi (SNP) OLR1’in 3’-terminal kısmındaki ekson 5’in alternatif eklenmesinde ve OLR1’in ekson 5’inden yoksun LOXIN transkript varyantının ekspresyonunda rol oynadığı rapor edilmiştir. 3’UTR (188C>T), intron 5 (IVS5-27G>T ve IVS5-70A>G) ve intron 4 (IVS4-14A>G, IVS4+27G>C, IVS4-73C>T) dahil olmak üzere bu 6 kodlamayan polimorfizm, haplotip blok bölgesinde yer almakta ve LOXIN ekspresyonunu değiştirerek KAH gelişme riskini etkilemektedir^12,13. Çalışmalar OLR1’in ateroskleroz ve tromboz patofizyolojisinde önemli bir rol oynayabileceğini bildirmiştir^13,14. OLR1 geninin 3’-UTR’sindeki bir polimorfizm olan 188C>T’nin KAH ile ilişkili olduğu bulunmuştur^13,15 ancak bu polimorfizmin damar hastalıklarıyla ilişkili olmadığını bildiren çalışmalar da mevcuttur^16-18. Ayrıca proenflamatuvar etkilere sahip olan C-reaktif proteinin (CRP) vasküler hücreler üzerinde proaterojenik ve protrombotik etkiler göstererek KAH patogenezinde rol oynayabileceği de rapor edilmiştir¹⁹. Bu bilgileri dikkate alarak Türk toplumunda KABG uygulanan hastalarda OLR1 188C>T polimorfizmi ile serum CRP ve lipid düzeylerini araştırmayı amaçladık.

MATERIALS AND METHODS

Subjects

This prospective case-control study included 109 patients (30 women, and 79 men) who had undergone the CABG surgery and 127 healthy controls (47 women, and 80 men). The median age of patients and controls was 58 and 50 years, respectively. Healthy persons without any history of cardiovascular events and without any symptoms of CAD were selected for the control group. The exclusion criteria included cancer, autoimmune, kidney, or hepatic disease. All study subjects were of Turkish origin and provided signed informed consent before the sample and data collection. This study was approved by the University of Health Sciences Turkey, İstanbul Training and Research Hospital Ethics Committee (no: 162, date: 20.05.2022). All procedures followed the ethical standards of the responsible committee on human experimentation (institutional and national) and/or the Helsinki Declaration of 1964 and later versions.

Blood Collection

Blood samples were collected in two tubes containing ethylenediaminetetraacetic acid (EDTA) after an overnight fast. One of the blood tubes containing EDTA was used for genotype analysis and stored as frozen at -80 °C until analysis. After centrifugation of the other tube at 400 x g for 10 minutes at 4 °C, plasma samples were separated into eppendorf tubes and frozen at -20°C until analysis.

Biochemical Measurements

The plasma concentrations of CRP were measured by nephelometric immunoassay using Dade Behring kits (BN II System Analyzer Dade Behring, Germany). In addition, plasma high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) levels were measured by automated colorimetric methods with commercially available kits (Cobas 8000, Roche Diagnostics GmbH, Mannheim, Germany).

DNA Isolation and Genotyping

Blood was drawn into EDTA-containing tubes for DNA isolation. The Roche DNA purification kit (Roche Diagnostics GmbH, Mannheim, Germany) was used to extract DNA from peripheral blood leukocytes in accordance with the manufacturer’s instructions. Isolated DNA samples were kept frozen at -80 °C. The ORL1 188C>T polymorphism was genotyped using standard polymerase chain reaction (PCR) procedures and restriction enzyme digestion. There were two primer pairs used: F: 5’-TGTCAACATTTTTGATTCTAGCTA-3’, and R: 5’-GTTCTCCATGTTCTGTCTTTCA-3’. The PCR mixture (20 μL total volume) consisted of 25 ng of gDNA, 10 pmol/μL of each primer, and 2 x PCR master mix solution (intron Biotechnology, Korea) which contents of 2.5 mM of each dNTPs, 2.5 U of i-Taq^TM DNA polymerase, 1 x of PCR reaction buffer and 1 x of gel loading buffer. A Techne Thermal Cycler (Applied Biosystems Gene Amp PCR System 9700, Singapore) was used to perform the PCR. PCR conditions were as follows: initial denaturation at 94 °C for 2 min, followed by 40 cycles of denaturation at 94 °C for 20 sec, annealing at 51 °C for 10 sec, and elongation at 72 °C for 30 sec. The final amplicon extension was performed at 72 °C for 5 min. PCR amplicons were separated by electrophoresis in 2% agarose gel and visualized by ethidium bromide staining. The 188C>T PCR product was 207 bp. The amplified PCR products were directly digested by the KpnI (Invitrogen Life Tecnologies Corporation, Carlsbad, CA, USA) restriction enzyme (10 units/µL) at 37 °C for overnight. After KpnI restriction, two fragments were obtained: 184 and 23 bp for the C allele and a single fragment of 207 bp for the T allele. The digested DNAs were separated on 3% agarose gel in 1 x Tris borate EDTA buffer followed by staining with ethidium bromide solution. The 188C>T genotypes were detected using a Polaroid camera and viewed under ultraviolet light.

Statistical Analysis

The Statistical Package for the Social Sciences statistic 21.0 program was used for the analyses of the patients and control values. Hardy-Weinberg equilibrium was tested by chi-square analysis. Genotype and allele frequencies were compared between cases and controls by chi-square analysis. Odds ratio and respective 95% confidence intervals evaluated the effects of any difference between the allelic and genotype distributions. The unpaired Student’s t-test (normally distributed variables) or Mann-Whitney U test (not normally distributed variables) were used for the comparison of other parameters. A value of p<0.05 was considered the minimum statistical significance.

RESULTS

The characteristics of patients undergoing CABG and the control groups were summarized in Table 1. There was no significant difference in terms of age, gender, body mass index (BMI), HDL-C, LDL-C, and systolic blood pressure between the patient and control groups (p>0.05). Cigarette smoking, alcohol use, family history, diabetes mellitus, CRP, hypertension, and diastolic blood pressure were increased in patients compared to the control group (p<0.001, p<0.001, p<0.001, p<0.001, p<0.001, p<0.001, and p<0.05, respectively).

We investigated the OLR1 3’-UTR 188C>T gene polymorphism, the frequencies of the genotypes in CABG patients and control groups were shown in Table 2. The distribution of TT, CT, and CC genotypes was 53.21%, 26.60%, and 20.18%, respectively, in the cases, and 46.45%, and 33.85%, and 19.69%, respectively, in the controls. No statistically significant difference was observed in the gene polymorphism between the two groups (p>0.05). Also, the distribution of T and C alleles was 66.51% and 33.48%, respectively, in the cases and 63.38 % and 36.61 %, respectively, in the controls (p>0.05) (Table 2).

To assess whether OLR1 3’-UTR 188C>T gene polymorphism had any effect on BMI, CRP, HDL-C, LDL-C, and blood pressure levels, we compared these parameters among the genotype groups in patients and controls (Table 3). The CRP levels were higher in patients with the TT, CT, and CC genotypes than in controls with the same genotypes (p<0.001, p<0.01 and p<0.05, respectively). Also, the systolic blood pressure levels were higher in patients with the CC genotype than in controls with the same genotype (p<0.05). The diastolic blood pressure levels were higher in patients with the TT genotype than in controls with the same genotype (p<0.05). No significant difference was detected in the comparison of the same genotypes of other parameters between the patient and control groups.

DISCUSSION

It has been reported that the human gene encoding the lectin-like oxidized LDL receptor, also called OLR1, is a good candidate for cardiovascular disease and is located in a chromosomal region associated with cardiovascular disorders¹⁰. Also, SNPs in the OLR1 gene have been reported to be associated with the risk of developing CAD¹². OLR1 3’ UTR 188C>T polymorphism can affect LOX-1 expression by altering its regulator binding site and thus modifying protein homeostasis²⁰. Therefore, in our study, OLR1 3’ UTR 188C>T polymorphism was investigated in patients who underwent CABG, and CRP, lipid, and blood pressure levels were evaluated in the same genotype distributions.

In the literature, studies investigating the OLR1 188C>T polymorphism in vascular diseases have reported conflicting findings^{13,15,16,18,21}. Mango et al.¹³ examined the 3’ UTR 188C>T polymorphism in patients with acute myocardial infarction in the Italian population and they reported that there was a significant change between the patient and control groups, and T allele carriage increased the risk of cardiovascular events. Similarly, Guo et al.²¹ observed that the frequency of the T allele in 188C>T polymorphism was significantly higher in patients with atherosclerotic cerebral infarction compared to healthy controls in the Chinese population. Novelli et al.¹⁵ confirmed the association of the 3’UTR 188C>T SNP with myocardial infarction (p<0.003). Cheng et al.²² studied a meta-analysis including 8 studies and reported that 3’UTR 188C>T increased CAD sensitivity. According to a meta-analysis study including findings of 11 researches suggested that variant genotype in the 188C>T polymorphism was associated with the increased risk of CAD²³.

However, there are also studies reporting any changes in OLR1 3’UTR 188C>T polymorphism in vascular diseases. Liu et al.²⁴ investigated the association between LOX-1 gene polymorphism, 3’UTR 188C>T, and cerebral infarction in the northern Chinese Han population. However, they did not find an association between 3’UTR 188C>T and cerebral infarction risk. Sentinelli et al.¹⁶ reported that there was no significant difference in the allele frequencies of the OLR1 gene between CAD and controls in the Italian population, and that the 3’ UTR 188C>T polymorphism was unlikely to play a role in the pathogenesis of CAD in the studied population. Similarly, Trabetti et al.¹⁷ did not find a statistically significant difference in the allele or genotype distribution of 3’UTR SNPs in CAD in the Italian population when compared to subjects without CAD. Kurnaz et al.¹⁸ reported that no correlation was observed between the 3’UTR 188C>T SNP and the presence of CAD in the Turkish population, and there was no difference in the comparison analysis between genotype groups and the mean values of cardiovascular risk factors. Tripathi et al.²⁵ reported that the 3’UTR 188C>T polymorphism in the North Indian population did not show a significant difference between CAD patients and healthy controls.

When the findings of our study were evaluated, it was observed that there was no significant change in the OLR1 3’UTR 188C>T genotype and allele distributions between CABG patients and healthy volunteers. The conflicting findings regarding the 3’UTR 188C>T polymorphism reported in the literature may be due to the limited size of the study populations, ethnic diversity of polymorphisms, and complex environmental factors.

In addition to genetic factors in the development of CAD, modifiable factors such as smoking, physical inactivity, overweight, uncontrolled stress, and unhealthy diet are also effective as well as non-modifiable factors such as advanced age, male gender, and race. Epidemiological studies report that risk factors such as high cholesterol level, hypertension, and diabetes mellitus also play a role in the development of atherosclerosis²⁶.

It is known that atherogenesis is promoted by high plasma and tissue levels of oxidized low-density lipoproteins (OxLDLs). Ox-LDLs increase the expression of proinflammatory genes, leading to monocyte recruitment to the vessel wall, and dysfunction of vascular endothelial cells. Ox-LDLs transform macrophages into foam cells that form atherosclerotic plaques²⁷.

Stancel et al.¹⁹ suggested that CRP and LOX-1 constituted a cyclic mechanism with ox-LDL in atherogenesis. CRP is an acute phase protein primarily synthesized by hepatocytes. It has been reported that CRP may play a direct role in promoting the inflammatory component of atherosclerosis. In addition, an upregulation of CRP levels was detected in plaque tissues². CRP is a ligand for OLR1, increases vascular permeability, impairs endothelium-dependent vasodilator function, and plays a role in monocyte-endothelial cell adhesion^19,27.

When the findings of our study were evaluated, it was noted that smoking, alcohol consumption, family history, hypertension, diabetes mellitus, and CRP levels increased in the CABG group compared to the control (Table 1). These data are compatible with the findings on risk factors affecting the development of atherogenesis. We also investigated the effect of the same genotype on BMI, HDL-C, LDL-C, CRP, systolic blood pressure, and diastolic blood pressure in the patient and control groups. CRP values were increased in all 3 genotypes compared to the control group, and the systolic blood pressure value in the CC genotype and the diastolic blood pressure value in the TT genotype were lower in the patient group compared to the control group (Table 3).

Unlike many other inflammatory mediators, CRP is not subject to diurnal fluctuation or biological variance. Therefore, CRP concentration seems to be proportional to the severity of the disease. However, the main limitation of CRP is its elevation in systemic inflammation, which may limit its use as a prognostic marker in postoperative patients²⁸. It is thought that the increase in CRP values in our patient group may be related to the CABG operation.

Study Limitations

It should be noted that the limitations of our study are its small sample size and its being a single-center study, which may have influenced the statistical power of our analysis.

TARTIŞMA

OLR1 olarak da adlandırılan lektin benzeri oksitlenmiş LDL reseptörünü kodlayan insan geninin, kardiyovasküler hastalıklar için iyi bir aday olduğu ve kardiyovasküler bozukluklarla ilişkili bir kromozomal bölgede yer aldığı rapor edilmiştir¹⁰. Ayrıca OLR1 genindeki SNP’ler de araştırılmıştır. KAH gelişme riski ile ilişkili olduğu bildirilmektedir¹². OLR1 3’ UTR 188C>T polimorfizmi, regülatör bağlanma bölgesini değiştirerek ve dolayısıyla protein homeostazisini değiştirerek LOX-1 ekspresyonunu etkileyebilir²⁰. Bu nedenle çalışmamızda KABG uygulanan hastalarda OLR1 3’ UTR 188C>T polimorfizmi araştırıldı, ayrıca CRP, lipid ve kan basıncı düzeyleri aynı genotip dağılımlarında değerlendirildi.

Literatürde damar hastalıklarında OLR1 188C>T polimorfizmini araştıran çalışmalarda çelişkili bulgular rapor edilmiştir^{13,15,16,18,21}. Mango ve ark.¹³ İtalyan popülasyonunda akut miyokard enfarktüsü geçiren hastalarda 3’ UTR 188C>T polimorfizmini incelemiş, hasta ve kontrol grupları arasında anlamlı değişiklik olduğunu, T aleli taşıyıcılığının kardiyovasküler olay riskini artırdığını bildirmişlerdir. Benzer şekilde Guo ve ark.²¹ 188C>T polimorfizminde T alelinin sıklığının, Çin popülasyonundaki sağlıklı kontrollerle karşılaştırıldığında aterosklerotik serebral enfarktüslü hastalarda anlamlı derecede yüksek olduğunu gözlemlemişlerdir²¹. Novelli ve ark.¹⁵ 3’UTR 188C>T SNP’nin miyokard enfarktüsü ile ilişkisini doğrulamışlardır (p<0,003). Cheng ve ark.²² 3’UTR 188C>T’nin KAH duyarlılığını arttırdığına dair 8 çalışmayı içeren bir meta-analiz bildirmişlerdir. On bir araştırmanın bulgularını içeren bir başka meta-analiz çalışmasında 188C>T polimorfizmindeki varyant genotipinin KAH riskinin artmasıyla ilişkili olduğu öne sürülmüştür²³.

Ancak damar hastalıklarında OLR1 3’UTR 188C>T polimorfizminde değişiklik bulunmadığını bildiren çalışmalar da mevcuttur. Liu ve ark.²⁴ Kuzey Çin Han popülasyonunda LOX-1 gen polimorfizmi, 3’UTR 188C>T ile serebral enfarktüs arasındaki ilişkiyi araştırmışlardır. Ancak 3’UTR 188C>T ile serebral enfarktüs riski arasında bir ilişki bulunmadığını bildirmişlerdir. Sentinelli ve ark.¹⁶ İtalyan popülasyonunda KAH ve kontroller arasında OLR1 geninin alel frekanslarında anlamlı bir fark olmadığını ve 3’ UTR 188C>T polimorfizminin çalışılan popülasyonda KAH patogenezinde rol oynama ihtimalinin düşük olduğunu bildirmişlerdir. Benzer şekilde Trabetti ve ark.¹⁷ İtalyan popülasyonunda KAH’taki 3’UTR SNP’lerin alel veya genotip dağılımında, KAH olmayan deneklerle karşılaştırıldığında istatistiksel olarak anlamlı bir fark bulamamışlardır. Kurnaz ve ark.¹⁸ Türk toplumunda 3’UTR 188C>T SNP ile KAH varlığı arasında korelasyon görülmediğini, genotip grupları arasında karşılaştırma analizinde ve kardiyovasküler risk faktörlerinin ortalama değerlerinde farklılık olmadığını bildirmişlerdir. Tripathi ve ark.²⁵ Kuzey Hindistan popülasyonundaki 3’UTR 188C>T polimorfizminin KAH hastaları ile sağlıklı kontroller arasında anlamlı bir fark göstermediğini bildirmiştir.

Çalışmamızın bulguları değerlendirildiğinde, KABG hastaları ile sağlıklı gönüllüler arasında OLR1 3’UTR 188C>T genotipi ve alel dağılımlarında anlamlı bir değişiklik olmadığı görüldü. Literatürde 3’UTR 188C>T polimorfizmi ile ilgili olarak bildirilen çelişkili bulgular, çalışma popülasyonlarının sınırlı olmasından, polimorfizmlerin etnik çeşitliliğinden ve karmaşık çevresel faktörlerden kaynaklanıyor olabilir.

KAH gelişiminde genetik faktörlerin yanı sıra sigara içme, fiziksel hareketsizlik, aşırı kilo, kontrolsüz stres, sağlıksız beslenme gibi değiştirilebilir faktörler ile ileri yaş, erkek cinsiyet, ırk gibi değiştirilemeyen faktörler de etkilidir. Epidemiyolojik çalışmalar, yüksek kolesterol düzeyi, hipertansiyon ve diyabet gibi risk faktörlerinin de ateroskleroz gelişiminde rol oynadığını bildirmektedir²⁶. Aterojenezin, oksitlenmiş düşük yoğunluklu lipoproteinlerin (OxLDL’ler) yüksek plazma ve doku seviyeleri tarafından desteklendiği bilinmektedir. Ox-LDL’ler proenflamatuvar genlerin ekspresyonunu artırarak damar duvarına monosit toplanmasına ve vasküler endotelyal hücrelerin fonksiyon bozukluğuna yol açmaktadır. Ox-LDL’ler makrofajları aterosklerotik plaklar oluşturan köpük hücrelerine dönüştürülmektedir²⁷.

Stancel ve ark.¹⁹ CRP ve LOX-1’in aterogenezde ox-LDL ile siklik bir mekanizma oluşturduğunu ileri sürmüştür. CRP, öncelikle hepatositler tarafından sentezlenen bir akut faz proteinidir. CRP’nin aterosklerozun enflamatuvar bileşenini teşvik etmede doğrudan rol oynayabileceği rapor edilmiştir. Ek olarak, plak dokularında CRP seviyelerinde bir artış tespit edilmiştir². CRP, OLR1 için bir liganttır, vasküler geçirgenliği arttırır, endotel bağımlı vazodilatör fonksiyonunu bozar ve monosit-endotel hücre adezyonunda rol oynamaktadır^19,27.

Çalışmamızın bulguları değerlendirildiğinde KABG grubunda kontrole göre sigara, alkol tüketimi, aile öyküsü, hipertansiyon, diyabet ve CRP düzeylerinin arttığı görüldü (Tablo 1). Bu veriler aterogenez gelişimini etkileyen risk faktörlerine ilişkin bulgularla uyumludur. Aynı genotipin hasta ve kontrol gruplarında VKİ, HDL-K, LDL-K, CRP, sistolik kan basıncı ve diyastolik kan basıncı üzerine etkisini de araştırdık. Her 3 genotipte CRP değerleri kontrol grubuna göre artmıştı, ayrıca hasta grubunda CC genotipinde sistolik kan basıncı değeri, TT genotipinde diyastolik kan basıncı değeri kontrol grubuna göre daha düşüktü (Tablo 3).

Diğer birçok enflamatuvar medyatörden farklı olarak CRP, günlük dalgalanmalara veya biyolojik değişikliklere tabi değildir. Bu nedenle CRP konsantrasyonu hastalığın şiddeti ile orantılı görünmektedir. Bununla birlikte, CRP’nin ana sınırlaması, sistemik enflamasyonun artmasıdır ve bu da postoperatif hastalarda prognostik bir belirteç olarak kullanımını sınırlayabilir²⁸. Hasta grubumuzdaki CRP değerlerindeki artışın KABG operasyonuna bağlı olabileceği düşünülmektedir.

Çalışmanın Kısıtlılıkları

Çalışmamızın örneklem büyüklüğünün küçük olması ve tek merkezli bir çalışma olması, analizlerimizin istatistiksel gücünü etkilemiş olabilir.

CONCLUSION

In conclusion, the results of the study indicate that, for our Turkish sample, OLR1 3’UTR 188C>T polymorphism may not be involved in susceptibility to atherosclerosis but traditional risk factors in atherosclerosis such as smoking, alcohol consumption, family history, hypertension, diabetes mellitus, and circulating CRP levels were increased in our CABG population. The OLR1 3’UTR188C>T and different OLR1 SNPs may need to be evaluated with regard to their single and combined effects at risk of atherosclerosis.