ÖZ
Amaç
Bu çalışma, Lactobacillus paracasei subsp. paracasei (L. paracasei) elde edilen postbiyotiklerin HepG2 hücre hattı üzerindeki antiproliferatif etkilerini ve Candida türlerine karşı antifungal aktivitelerini değerlendirmeyi amaçlamıştır.
Gereç ve Yöntemler
L. paracasei izolatlarından hücresiz süpernatantlar (CFS) elde edilmiş ve HepG2 hücre hatları üzerindeki sitotoksik etkileri MTT testi kullanılarak değerlendirilmiştir. Belirlenen inhibitör konsantrasyon 50 (IC50) hücreler üzerinde floresan boyama ve hücre döngüsü analizleri gerçekleştirilmiştir. Postbiyotiklerin antifungal aktiviteleri, Candida albicans (C. albicans) ATCC 90028 ve Candida glabrata (C. glabrata) ATCC 2950 suşları üzerinde minimum IC50 ve minimum fungisidal konsantrasyon değerleri belirlenerek değerlendirilmiştir.
Bulgular
Canlı ve ısıyla inaktif edilmiş L. paracasei CFS’lerinin HepG2 hücreleri üzerinde anlamlı sitotoksik etkiler gösterdiği bulunmuştur. İnaktif edilmiş CFS, daha düşük IC50 değeri ile daha güçlü antiproliferatif etki oluşturmuştur (canlı: %20,13; inaktif: %10,81 standart sapma). Akridin oranj/propidyum iyodür boyaması apoptotik hücrelerde artış olduğunu gösterirken, hücre döngüsü analizi Sub-G fazında anlamlı bir artış olduğunu ortaya koymuştur (kontrol %0,67; canlı CFS %6,47; inaktif CFS %12,67). Antifungal testlerde her iki CFS’nin de C. albicans ve C. glabrata’ya karşı etkili olduğu belirlenmiştir. İnaktif CFS (%6,25) C. albicans üzerinde daha güçlü bulunurken, canlı CFS’nin (%12,5) C. glabrata üzerinde daha etkili olduğu saptanmıştır.
Sonuç
Bu çalışma, L. paracasei CFS’lerinin antikanser ve antifungal etkilere sahip olduğunu göstermektedir. Bu etkilerin CFS formuna ve hedef maya türüne bağlı olarak değişmesi, postbiyotik yanıtın biyolojik olarak yönlendirildiğini ve özgül olduğunu düşündürmektedir. CFS’nin aktif bileşenlerinin aydınlatılması, yeni postbiyotik temelli tedavi stratejilerinin geliştirilmesine önemli katkılar sağlayacaktır.
Anahtar Kelimeler:
L. paracasei, HepG2, Candida, antikanser, antifungal
Kaynaklar
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